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Produkty / AF NutriStem™ hESC XF - Xeno-Free medium for human ES & iPS Cell culture, without HSA, 500ml

AF NutriStem™ hESC XF - Xeno-Free medium for human ES & iPS Cell culture, without HSA, 500ml

Nr kat. 05-102-1A
Defined, xeno-free, serum-free medium, designed to support the growth of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC).

Producent: Biological Industries

CENA NETTO
620.00 zł
CENA BRUTTO
669.60 zł (z 8% VAT)
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OPIS PRODUKTU


Traditional human Embryonic Stem Cells culture methods require the use of mouse or human fibroblast feeder layers, or feeder-conditioned medium. These culture methods are labor-intensive, hard to scale up, and it is difficult to maintain human ES cells undifferentiated due to undefined conditions.


NutriStem™ media were developed by Biological Industries with a leading group in stem cell research, to enable the maintenance and expansion of human ES and iPS cells with feeder cells or on feeder-independent culture.


Human ES cells (i.e., H9.2, I6, I3.2, H1) cultured in NutriStem™ media displayed:
The ability to self-renew, by expansion in feeder-free culture conditions (Matrigel), on human-feeder layer (HFF) or on Mouse feeder cells (MEFs) 


Expression of hESC specific transcription factors and antigens Pluripotency by the ability to differentiate into the three germ layers (endoderm, ectoderm and mesoderm) in-vitro by EB formation and in-vivo by teratoma formation in SCID mice
and Human ES cell normal phenotype and genotype 


NutriStem™ human ES & iPS cell culture media provides:
Consistent media performance and predictable cellular behavior derived from a defined xeno-free formulation
Increased reproducibility shown in long-term growth of over 50 passages
in eliminating the need to prepare feeder cells when choosing to culture on feeder-free layer
Cost efficiency obtained in low human recombinant growth factor concentrations (rh bFGF, rh TGFβ)

Features
Albumin-Free
Optimized for feeder-dependent culture (i.e. HFF and MEF)
Superior performance on HFF feeder layer
Ready-to-use
When culturing on feeder-free layer (i.e. Matrigel) an addition of 5% of Bio-Pure Human Serum Albumin (HSA solution, 10%) (Cat.# 05-720-1) is required.

ZAŁĄCZNIKI PDF
AF NutriStem™ hESC XF - Xeno-Free medium for human ES & iPS Cell culture, without HSA, 500mlAF NutriStem™ hESC XF - Xeno-Free medium for human ES & iPS Cell culture, without HSA, 500mlAF NutriStem™ hESC XF - Xeno-Free medium for human ES & iPS Cell culture, without HSA, 500mlAF NutriStem™ hESC XF - Xeno-Free medium for human ES & iPS Cell culture, without HSA, 500ml, 05-102-1A
GENOS, ul. Inowrocławska 9/132, 91-020 Łódź, tel. 42 611 63 11, tel./fax 42 611 63 12, genos@genos.com.pl