- Peripheral Blood Karyotyping Medium with PHA, 100 ml
- Aquaguard-1 - Roztwór do dezynfekcji inkubatorów, 100ml
- Cell Proliferation Kit XTT, 1000 assays
- Pipety serologiczne 5 ml, TPP
- Butle do hodowli 25 cm/ Filter Cap, 10 szt.
- Butle do hodowli 75 cm/ Filter Cap, 5 szt.
- Cell Freezing Medium with DMSO, 20ml
- Peripheral Blood Karyotyping Medium with PHA, 500 ml
- Penicillin-Streptomycin solution, 1x, 100 ml
- EZ-PCR Mycoplasma Test Kit, 20 assays
NutriStem™ hESC XF - Xeno-Free medium for human ES & iPS Cell culture, with HSA, 500ml
Producent: Biological Industries
Traditional human Embryonic Stem Cells culture methods require the use of mouse or human fibroblast feeder layers, or feeder-conditioned medium. These culture methods are labor-intensive, hard to scale up, and it is difficult to maintain human ES cells undifferentiated due to undefined conditions.
NutriStem™ hESC XF medium was developed by Biological Industries with a leading group in stem cell research, to enable the maintenance and expansion of human ES and iPS cells with feeder cells or on feeder-independent culture.
Human ES cells (i.e., H9.2, I6, I3.2, H1) cultured in NutriStem™ XF hESC medium displayed:
The ability to self-renew, by expansion in feeder-free culture conditions (Matrigel), on human-feeder layer (HFF) or on Mouse feeder cells (MEFs)
Expression of hESC specific transcription factors and antigens
Pluripotency by the ability to differentiate into the three germ layers (endoderm, ectoderm and mesoderm) in-vitro by EB formation and in-vivo by teratoma formation in SCID mice andHuman ES cell normal phenotype and genotype
NutriStem™ human ES & iPS cell culture media provides:
Consistent media performance and predictable cellular behavior derived from a defined xeno-free formulation
Increased reproducibility shown in long-term growth of over 50 passages
Time saving in eliminating the need to prepare feeder cells when choosing to culture on feeder-free layer
Cost efficiency obtained in low human recombinant growth factor concentrations (rh bFGF, rh TGFβ)
Contains human serum albumin (HSA)
Complete ready-to-use (no additions required).
Contains alanyl glutamine, and does not contain antibiotics.
Optimized for feeder-free culture (Matrigel)
Good performance on feeder-dependent culture (i.e. HFF and MEF)
Superior performance on Matrigel
NutriStem® hPSC XF Medium Technical Manual
Weekend-free culture of human pluripotent stem cells on LN-521 (BioLamina)
NutriStem® hPSC XF Medium Product Sheet
Application Note: Single Cell Passaging with NutriStem® hPSC XF on Laminin-521 without the Need for ROCK Inhibitor
Technical Resources: Passaging Methods for hPSCs under Feeder-Free Conditions