Defined, xeno-free, serum-free culture medium for hPSC optimized for vitronectin.
NutriStem® V9 XF medium is a defined, xeno-free, serum-free medium designed to support the growth and expansion of human pluripotent stem cells (hPSC), using vitronectin and enzyme-free passage using EDTA.
It contains only the essential components required for long-term maintenance of hES and hiPS cells.
NutriStem® V9 XF medium shows superior proliferation rates during long-term cultures, while maintaining the pluripotency of the cells.
NutriStem® V9 XF medium allows culture of hPSC in vitronectin pre-coated culture, as well as direct addition of vitronectin to the medium (no need for pre-coating).
Maintains human ES and iPS cell pluripotency at high passage
Superior results using Vitronectin, may be used with Matrigel coated plates
Save time and skip the time consuming precoating step!
Defined, serum-free, xeno-free
Produced under cGMP
Supports long-term expansionLong weekend-free (up to 60 hoursCytokine- free basal medium, applicable for EBs formation, reprogramming, and differentiation
The proteins used: HSA (Human Serum Albumin), rh bFGF, rh TGFβ, human transferrin and recombinant human insulin.
Contains Alanyl glutamine.
Does not contain antibiotics.
Each lot is tested for performance using H1 hESC by expansion of the cells for 3 passages,
IF staining for pluripotency marker, and morphology.
Other tests include sterility, pH, osmolality and endotoxins levels.