Soybean Trypsin Inhibitor
50-Fold Concentrate 5mg per ml
In a serum-free culture environment, the cells must be separated by rapid centrifugation or by utilizing trypsin inhibitors such as Soybean Trypsin Inhibitor (SBTI).
SBTI is a single polypeptide that forms a stable, stoichiometric, enzymically inactive complex with trypsin, thereby reducing the availability of trypsin by somewhat binding chymotrypsin.
With Biological Industries' Soybean Trypsin Inhibitor Solution, any excess Crystalline Trypsin Solution (Cat. No. 03-047-1) may be completely neutralized, thereby avoiding the use of serum for this purpose.
The cells may then be re-suspended successfully in a suitable growth medium.
Stored and shipped at -20ºC.
Before use, the solution is diluted by a factor of 50 with sterile PBS solution, and can then be stored at 4ºC for up to 60 days.
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