[Intended Use] This reagent is suitable for nucleic acid staining in in situ hybridization detection system.
[Principle] The main component of in situ hybridization blue staining solution is 4,6-diamidino-2-phenylindole (DAPI), which is a blue fluorescence dye that can penetrate cell membrane and bind to double-stranded DNA. Under ultraviolet light excitation or light of a fluorescence microscope, the nucleic acid emits blue fluorescence.
[Instructions] 1. Drop 10-20µL in situ hybridization blue staining solution into the tissue or cell area after slide in situ hybridization. 2. Cover the sample with a cover slide to ensure that the dye solution covers the sample evenly and avoid bubbles as much as possible. 3. Keep away from light at room temperature for 3-5 minutes. 4. Observation and photography under fluorescence microscope; 5. For long-term storage, use a nail polish or other adhesive to seal around the coverslip.